ABSTRACT Given the above, the objective of this study was to evaluate the bromatological, mineral and bioactive compounds of blackberry grown in a subtropical region. Blackberries fruits (Rubus sp.), Grown in an orchard of Unioeste, Campus Marechal Cândido Rondon (Paraná, Brazil), were used. Immediately after harvest, the fruits were taken to the Food Technology Laboratory for chemical analysis and bioactive compounds. The fruit samples for the analysis of reducing, bromatological and mineral sugars were frozen and sent to private laboratories. Hybrids are more perishable than cultivars. There is variation in color among the cultivars and hybrids studied. Hybrids and cultivars of black mulberry have a high content of ascorbic acid and fibers, with emphasis on the cultivar Tupy (75.0 mg 100 mL-1 and 7.23, respectively). Higher pH is verified in the cultivars Tupy and Arapaho (3.22 and 3.24, respectively). The Arapaho cultivar has fewer acid fruits (0.25 g 100 g-1), SS/total acidity ratio (36.88) and reducing sugar content (8.28 g 100g-1). Blackberry fruits are a rich source of bioactive compounds, such as cv. Chickasaw obtaining a higher content of total phenolic compounds (1368.84 mg EAG100mL-1) and the Boysenberry hybrid a greater amount of anthocyanin (5.11mg Ci-3-Gly g -1). The Chickasaw cultivar has a higher lipid content (4.59). There is no difference in moisture content, dry biomass and fruit firmness.

ABSTRACT The objective of this work was to evaluate asepsis protocols, composition and concentration of culture media in the in vitro establishment of three physalis species. In experiment I, seeds of 3 species were used x 3 asepsis protocols. In experiment II, 3 culture media and explants 3 species were used. In experiment III, explants of 2 species x 4 concentrations of MS culture medium were used. In experiment IV, explants of 2 species x 4 sucrose concentrations were used. For experiments II, III and IV after 30 days were evaluated some phytotechnical parameters. The experimental design was completely randomized, in a 3 x 3 (experiments I and II) and 2 x 4 (experiments III and IV) factorial scheme. Protocols II and III were appropriate for P. peruviana germination, and I and III for P. minima, the three protocols were efficient for controlling fungi and bacteria. The MS is the most suitable for the in vitro establishment of three species. The culture medium at 100% concentration obtained better values in the in vitro development parameters evaluated for the species P. peruviana and P. minima. Sucrose concentrations close to 50 and 20 g L-1 favored the establishment of P. peruviana and P. minima.

ABSTRACT The objective of this study was to evaluate the potential of rooting of podocarpos cuttings in function of the number of leaves, concentrations of IBA and phytorregulators, application methods and cutting season. Two experiments were carried out under weaning conditions, in a factorial scheme containing 3 replicates of 15 cuttings, the first with 4 concentrations of IBA, 3 leaves at the cuttings and 2 seasons. These were removed in August/2016 and January/2017, prepared with 10 cm in length and immersed in solutions for 10 s. In the second experiment three types of phytoregulators and 2 application forms were tested (slow and quick immersion). Cuttings were prepared in the same manner as in the first experiment, maintaining 4 leaves. The bases of the cuttings were immersed for 10 s and 24 h in the solutions, then taken to root. After 100 days of experimentation, it was concluded that cuttings collected in the summer had a higher percentage of rooting, 4 leaves and IBA concentration should be maintained between 750 and 800 mg L-1. The best form of application of phytoregulator is by rapid immersion.

Resumo Como a região oeste do Paraná é considerada subtropical, com altas temperaturas no verão, os híbridos de Encyclia são comercializados pela beleza de suas flores e durabilidade. No entanto, não existem estudos envolvendo plantas em vasos, tipos de substratos e fertilizantes, além dos híbridos para o gênero Encyclia. Diante do exposto, o objetivo deste trabalho foi avaliar o desenvolvimento vegetativo e o florescimento de Encyclia com base na adubação e no substrato.O experimento foi conduzido em um produtor comercial, de janeiro a setembro de 2017. As mudas permaneceram em ambiente protegido com 50% de sombreamento. O delineamento experimental foi em blocos casualizados, em esquema fatorial 4 x 5 (substratos x intervalos de fertilização), com 4 repetições por tratamento e 4 vasos por repetição. O lote escolhido era oriundo de semeadura in vitro, com o híbrido Encyclia “Kropp” x E. atropurpurea. Após cinco meses da última fertilização, foram realizadas avaliações relacionadas ao desenvolvimento vegetativo e floração. Os substratos à base de esfagno apresentaram melhores resultados no florescimento. As adubações orgânicas com Bokashi aos 45 dias após o transplante (DAT) favorecem a floração e o desenvolvimento vegetativo do híbrido de Encyclia, ocorrendo em vasos contendo substratos à base de esfagno e com adubações orgânicas acima de 60 DAT.

Abstract Due to the fact that the western region of Paraná is considered subtropical, with high temperatures in the summer, the hybrids of Encyclia are commercialized by the beauty of its flowers and durability. However, there are no studies involving potted plants, substrate types and fertilizers, as well as hybrids, for the genus Encyclia. Thus, the objective of this work was to evaluate the vegetative development and flowering of Encyclia based on fertilization and substrate. The experiment was conducted in a commercial orchard, from January to September/2017. The seedlings remained in a protected environment, with 50% shading. The experimental design was randomized blocks, in a 4 x 5 factorial scheme (substrates x fertilization intervals), with 4 replicates per treatment, and 4 pots per replicate. The lot chosen was sowed in vitro, with the hybrid Encyclia “Kropp” x E. atropurpurea. After five months of the last fertilization, the evaluations related to vegetative development and flowering were carried out. Substrates based on sphagnum presented better results in flowering. Organic fertilizations with Bokashi at 45 days after transplant (DAT) favor flowering and vegetative development of the Encyclia hybrid, which occurs in pots containing sphagnum-based substrates and with organic fertilizers above 60 DAT.

ABSTRACT The germination process of the Annona sylvatica A.St.-Hil becomes difficult because the seeds have physical and physiological numbness. Although, the objective was to evaluate the effect the gibberellic acid can have in overcoming this physiological process. So that, aimed to evaluate the effect of the gibberellic acid in the overcoming dormancy of araticum seeds. Two different projects were developed simultaneously (plants germination in the laboratory and emergence of seedlings in seed). At the laboratory, the experimental design used was completely casualized in factorial scheme 4 x 4. Evaluating the physiological aspect of the germination and vigor. The germination of the seeds presented superior performance when they were immersed in 1200 mg.L-1 of GA3, in 24 h of imbibition, reaching 67% of germination. In the emergence of seedlings in seed, the experimental design used was to randomize blocks in factorial scheme 4 x 5 (four accesses x five periods of immersion). After 110 days, evaluating physiological aspects of emergence and vigor. The emergence varied between 32 to 45% (accesses A1 and A3) in a period of 15 to 24 h (accesses A1 and A3 or A4). High concentration and longer period of soaking in gibberellic acid promote a bigger germination of the araticum seeds. There is a difference between the accesses of the species, necessary to evaluate before being collected, and in the present study the accesses A3 is promising for harvest.

RESUMO O processo germinativo da Annona sylvatica A.St.-Hil. torna-se difícil porque as sementes apresentam dormência física e fisiológica. Porém, o ácido giberélico pode atuar na superação desse processo fisiológico. Assim, objetivou-se avaliar o efeito do ácido giberélico na superação de dormência em sementes de araticum. Foram desenvolvidos dois trabalhos distintos e simultaneamente (germinação de sementes em laboratório e emergência de plântulas em sementeira). Em laboratório, o delineamento experimental utilizado foi inteiramente casualizado em esquema fatorial 4 x 4 [quatro concentrações de ácido giberélico (0, 400, 800 e 1200 mg.L-1 x quatro períodos de imersão na solução (0, 8, 16 e 24 h)], avaliando-se os aspectos de germinação e vigor. A germinação de sementes apresentou desempenho superior quando foram embebidas em 1200 mg.L-1 de GA3 em 24 h de embebição, alcançando 67% de germinação. Na emergência de plântulas em sementeira, o delineamento experimental utilizado foi em blocos casualizados em esquema fatorial 4 x 5 [quatro acessos x cinco períodos de imersão (0, 18, 20, 22 e 24 h). Após 110 dias, avaliou-se aspectos fisiológicos de emergência e vigor. A emergência variou entre 32 a 45% (acessos A1 e A3) num período de 15 a 24 horas (acessos A1 e A3 ou A4). Alta concentração e maior período de embebição em ácido giberélico promove maior germinação de sementes de araticum. Existe diferença entre os acessos da espécie, necessário avaliação das mesmas antes da coleta, e no presente estudo o acesso A3 é promissor para coletas.

RESUMO: A propagação assexuada de coníferas, como o Podocarpus macrophyllus, apresenta dificuldades. Entre os fatores que podem tornar mais eficientes a propagação assexuada tem-se o tipo da estaca utilizada, lesões na base da estaca e uso de fitoreguladores, como o ácido indolbutírico (AIB). Objetivou-se determinar o tipo de lesão a ser efetuado na base da estaca e o tipo de estaca bem como método de veiculação do AIB na base da estaca e a concentração do AIB visando obter melhor enraizamento para a espécie. Dois experimentos foram realizados, no período de março a julho de 2018, em blocos casualizados e esquema fatorial, sendo o primeiro 2 x 4 [aplicação AIB em solução e em talco x concentração de 0, 500, 1000 e 1500 mg L-1 de ácido indolbutírico (AIB)] e o segundo 2 x 3 [estaca basal e apical x lesões na base da estaca do tipo bisel, cunha e horizontal].Em ambos experimentos as estacas utilizadas foram dispostas em canteiro de alvenaria contendo areia de textura média. Após 120 dias de experimentação conclui-se que o uso de estacas basais com lesões na base da estaca em cunha e tratadas com AIB em solução na concentração de aproximadamente 550 mL L-1favorecem a porcentagem de enraizamento.

ABSTRACT: The asexual propagation of conifers, such as Podocarpus macrophyllus (Thunb.) Sweet, faces difficulties. Among the factors that can improve the efficiency of asexual propagation are the types of cuttings used and lesions performed at the base of the cuttings, and the use of plant regulators, such as indolebutyric acid (IBA). This study determined the types of lesions to be performed at the cutting base, the type of cuttings used, the method to convey IBA at the cutting base, and the concentration of IBA required to obtain a proper rooting condition for the species. Two experiments were performed from March to July, 2018, in randomized blocks and with a factorial scheme, as follows: 2 x 4 (IBA application in solution and talc x IBA at concentrations of 0, 500, 1,000, and 1,500 mg L-1 IBA); and 2 x 3 (basal and apical cuttings x bevel, wedge, and horizontal lesions at the cutting base). In both experiments, the cuttings used were arranged on a masonry bed containing medium-textured sand. After 120 days of experimentation, the study concluded that the rooting percentage was favored by the use of basal cuttings with wedge lesions at the cutting base and treated with IBA in solution at a concentration of approximately 550 mg L-1.

ABSTRACT The purpose this study was to evaluate the vegetative development, yield and quality of strawberry cultivars of short and neutral days as a function of soil cover. Two experiments were carried out in a protected environment. The experimental design used in both experiments consisted of randomized blocks, with subdivided plots. Harvests were carried out every two days, when the berries presented 75% of the red coloration of the epidermis, with evaluation of yield, number of fruits/plant, and fresh biomass of the fruits. For fruit quality, the pH, SS, titratable acidity, SS/TA ratio and fruit staining were evaluated. The contents of chlorophyll a and b, chlorophyll a + b and the chlorophyll a/b ratio in the leaves were also measured. At the end of the productive cycle, destructive evaluations of leaf number, leaf area and dry biomass of shoot were carried out. Cultivation on white plastic soil coverage presented plants with lower vegetative development, higher yield, and fruit size. Among the cultivars of short days, Camino Real presented bigger and more attractive fruits regarding the appearance of color of epidermis. For neutral day cultivars, Aromas obtained higher yield.

ABSTRACT The purpose of this study was to evaluate the phenological behavior and agronomic potential of blackberry varieties and hybrids in a subtropical region. Blackberry seedlings of four varieties and two hybrids were purchased in April 2015 in the form of stem cuttings and transplanting in July 2015. Winter pruning was carried out on August 18, 2016 and 2017. The experimental design was composed of a randomized complete block design, containing four varieties and two hybrids, four blocks and five useful plants per experimental unit. The harvest was performed every two days to the determination of fresh biomass, longitudinal and transverse diameter, volume, number of fruits per plant, yield and estimated productivity. The average duration of the phenological cycles in the two harvests varies between 109 and 162 days and 114 and 148, respectively. Hybrids Boysenberry and Olallie presented a shorter cycle in both harvests. In the 2016/2017 crop, the harvest lasted from 39 to 88 days. In the 2017/2018 harvest, it lasted 23 to 57 days, starting in mid-November through early January. The Tupy and Chickasaw cultivars were the most productive in the first crop. In the second crop, the Tupy and Navaho cultivars were prominent.

RESUMO: A framboseira-negra possui potencial de cultivo no Brasil pelo fato dessa fruteira produzir altas quantidades de frutos que conferem uma geleia de alta qualidade. No entanto, a sua propagação é um entrave no estabelecimento de plantios comerciais. Diante do exposto, objetivou-se, com o presente trabalho, avaliar a utilização da estaquia caulinar e ácido indolbutírico na propagação assexuada de framboeseira-negra. Foram realizados dois experimentos, sendo conduzidos em condições de telado. Estacas caulinares foram retiradas de pomar didático de plantas matrizes de quatro anos, as quais constituíram os experimentos. O primeiro experimento foi elaborado em esquema fatorial 3x2, sendo as estacas retiradas de três regiões do ramo da planta matriz (apical, mediana e basal) x dois tratamentos com ácido indolbutírico (sem AIB e 2000mg L-1 de AIB), contendo quatro repetições e quinze estacas por repetição. O segundo experimento constituiu-se de esquema fatorial 4x2, sendo quatro tamanhos de estacas (5, 10, 15 e 20cm) x dois tratamentos com ácido indolbutírico (sem AIB e 2000mg L-1 de AIB), contendo quatro repetições e quinze estacas por repetição. Após oitenta dias, avaliaram-se a percentagem de estacas enraizadas (%); número médio de raízes e de brotações por estacas; comprimento da maior raiz (cm) e biomassa fresca e seca das brotações (g). Melhores resultados na formação do sistema radicular foram observados em estacas caulinares medianas e apicais. Estacas caulinares entre 10 e 20cm obtiveram melhores resultados para enraizamento. Não há necessidade de utilização de AIB no enraizamento de estacas caulinares de framboeseira-negra.

ABSTRACT: The framboseira-negra has a potential for cultivation in Brazil, due to the fact that this fruit produces high quantities of fruits, which confer a high quality jelly. However, their propagation is an obstacle to the establishment of commercial plantations. Production and expansion of a fruit is directly linked to the propagation method and the quality of its development. Given the above, the aim of the present study was to evaluate the use of stem cuttings and IBA in asexual propagation of black raspberry. Two experiments being conducted in greenhouse conditions were performed. Stem cuttings were taken from didactic orchard, with matrix plant of four years, which were the experiments. The first experiment was carried out in factorial 3x2, with cuttings taken from three regions of the plant branch matrix (apical, median and basal) x two treatments with IBA (without IBA and 2000mg L-1 IBA), containing four repetitions and fifteen cuttings per repetition. The second experiment consisted of a 4x2, with four sizes of cuttings (5, 10, 15 and 20cm) x two treatments with IBA (without IBA and 2000mg L-1 IBA), with four replications and fifteen cuttings by repetition. After eighty days, the percentage of rooted cuttings (%), average number of roots and shoots for cuttings, the longest root length (cm) and fresh and dry biomass of shoots (g) were assessed. Best results in the formation of root system were observed in middle and apical stem cuttings. Stem Cuttings between 10 and 20cm obtained better results for rooting. There is no need to use IBA rooting of cuttings of black raspberry.

RESUMO: A propagação comercial de roseiras é realizada assexuadamente, pelo método de estaquia. O material genético e o substrato são fatores que interferem no processo rizogênico das estacas. Diante do exposto, objetivou-se com o presente trabalho avaliar o efeito do substrato sobre o enraizamento de estacas de roseiras Tineke e Natal Briar. Foram utilizados ramos floríferos de plantas matrizes cortadas em bisel no ápice e na base, com aproximadamente 8cm e duas gemas, sendo retirada a folha basal. O delineamento experimental utilizado foi inteiramente casualizado, em um fatorial 2 x 3 (duas cultivares, Tineke e Natal Briar x três substratos, casca de arroz carbonizada, fibra de coco e TN Gold ® ), contendo quatro repetições de 15 estacas por repetição. A base das estacas foi imersa por cinco segundos em solução de 2000mg L-1 de IBA (ácido indol butírico). As estacas foram colocadas em bandejas com 24 células previamente preenchidas com substrato e depositadas no chão em túnel plástico transparente, construído dentro da estufa. Após 50 dias de experimentação avaliaram-se as estacas enraizadas (%), brotadas (%) e mortas (%), comprimento das raízes (cm), biomassa fresca e seca da parte aérea e raízes (g). A porcentagem de enraizamento da cultivar Natal Briar foi de 75%, superior à cultivar Tineke, que obteve 39% de estacas enraizadas. Melhores substratos para a estaquia de roseiras Tineke e Natal Briar são a CAC e fibra de coco que proporcionaram o enraizamento de 81 e 67% das estacas respectivamente.

ABSTRACT: The commercial propagation of rose bushes is carried out asexually by the cutting method. The genetic material and the substrate are factors that interfere in the rhizogenic process of the cuttings. Thus, the aim of this work was to evaluate the effect of the substrate on the rooting of cuttings of two hybrid rose bushes. Floriferous branches of matrix plants were cut at the apex and at the base, approximately 8cm long and with two buds, and the basal leaf was removed. The experimental design was completely randomized, in a 2 × 3 factorial scheme (two cultivars, Tineke and Natal Briar × three substrates, carbonized rice husk, coconut fiber and TN Gold®) containing four replicates of 15 cuttings per replicate. The base of the cuttings was immersed for five seconds in IBA 2000mg L-1 solution (indole butyric acid). The cuttings were placed in trays with 24 cells previously filled with substrate and deposited on the floor in a transparent plastic tunnel, built inside a stove. After the 50-day experiment, rootstocks (%), sprouts (%) and dead (%), root length (cm), fresh and dry shoot biomass and roots (g) were evaluated. The rooting percentage of the Natal Briar cultivar was 75%, thus superior to the Tineke cultivar, which had a rooting percentage of 39%. The best substrates for the Tineke and Natal Briar rose cuttings are CAC and coconut fiber, which provided rooting percentages of 81% and 67% of the cuttings, respectively.